KLOTH, S and SCHMIDBAUER, A and KUBITZA, M and WEICH, HA and MINUTH, WW (1994) DEVELOPING RENAL MICROVASCULATURE CAN BE MAINTAINED UNDER PERFUSION CULTURE CONDITIONS. EUROPEAN JOURNAL OF CELL BIOLOGY, 63 (1). pp. 84-95. ISSN 0171-9335,
Full text not available from this repository.Abstract
The cortex corticis of the neonatal rabbit kidney consists of developing nephrons, vessels, collecting duct ampullae and the nephrogenic mesenchyme. Inductive interactions between embryonic mesenchyme and collecting duct ampullae lead to the coordinated development of the nephrons and the collecting duct system. The factors regulating nephrogenesis and vascular development within this tissue region are unknown. In order to analyze the hormonal regulation of vascular development an organotypic culture system was established. Cortex explants from neonatal rabbit kidneys were prepared, mounted in a set of holding rings and cultured under serum-free conditions for 14 days in conventional culture plates or under permanent medium perfusion in a newly developed culture container. The detection of endothelial cells was carried out by means of two monoclonal antibodies. Within the renal cortex corticis EnPo 1 detected developing vasculature as,veil as podocytes and a subset of mesenchymal cells. EC1 displayed exclusive specificity for endothelial cells. The antibody did not discriminate between arteries and reins. Endothelial cells of different developmental stages were labeled with the same intensity. A combination of both antibodies allowed the discrimination between developing endothelial cells and podocytes. Following 14 days of culture under permanent medium ex change, excellent tissue preservation as well as endothelial cell proliferation was observed in cortex explants. In contrast, tissue kept in stationary culture revealed a high degree of disintegration. Endothelial antigen expression was also severely disturbed. Tis sue maintenance under stationary conditions was improved by the application of a hormone mixture consisting of aldosterone and 1,25-hydroxyvitamin D3. However, the high degree of spatial organization shown by de,eloping endothelial cells in vivo was maintained exclusively in explants cultured in the presence of hormone under permanent perfusion.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL GROWTH-FACTOR; EMBRYONIC KIDNEY; ANGIOGENESIS FACTOR; TUMOR ANGIOGENESIS; CELL-CULTURES; EXPRESSION; MORPHOGENESIS; INVITRO; DIFFERENTIATION; NEPHROTOXICITY; ANGIOGENESIS; MONOCLONAL ANTIBODY; CELL CULTURE; KIDNEY; DEVELOPMENT |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53473 |
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