ADIPOGENIC ACTIVITIES IN COMMERCIAL PREPARATIONS OF FETUIN

BACHMEIER, M and LOFFLER, G (1994) ADIPOGENIC ACTIVITIES IN COMMERCIAL PREPARATIONS OF FETUIN. HORMONE AND METABOLIC RESEARCH, 26 (2). pp. 92-96. ISSN 0018-5043,

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Abstract

Fetuin is frequently in use as a serum substitute especially during the culture of differentiating preadipocyte cell lines. We show here, that the commercially available crude Pedersen fetuin contains a factor permissive for adipose conversion. It can be separated from fetuin by dialysis at acid pH. When 3T3-L1 cells are cultured in the presence of purified Pedersen fetuin, they fail to undergo adipose conversion unless this permissive adipogenic factor is added to the culture medium. Insulin-like growth factor I (IGF-I) and growth hormone (GH) are not able to replace the factor.

Item Type: Article
Uncontrolled Keywords: ADIPOSE-TISSUE DEVELOPMENT; GROWTH FACTOR-I; PRECURSOR CELLS; BOVINE FETUIN; 3T3-L1 CELLS; C-FOS; DIFFERENTIATION; SERUM; CONVERSION; HORMONE; 3T3-L1 PREDIPOCYTES; DIFFERENTIATION FETUIN
Depositing User: Dr. Gernot Deinzer
Last Modified: 19 Oct 2022 08:41
URI: https://pred.uni-regensburg.de/id/eprint/53477

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