SCHUTZ, M and ZIRNGIBL, S and LECOUTRE, J and BUTTNER, M and XIE, DL and NELSON, N and DEUTZMANN, R and HAUSKA, G (1994) A TRANSCRIPTION UNIT FOR THE RIESKE FES-PROTEIN AND CYTOCHROME-B IN CHLOROBIUM-LIMICOLA. PHOTOSYNTHESIS RESEARCH, 39 (2). pp. 163-174. ISSN 0166-8595, 1573-5079
Full text not available from this repository.Abstract
A transcription unit petCB from Chlorobium limicola is described. The leading gene petC codes for a Rieske FeS-protein of 19.04 kDa with 181 amino acid residues. The following gene petB codes for a cytochrome b of 47.48 kDa with 428 amino acid residues. The transcription unit lacks a third gene pet-A for cytochrome c(1) or -f, which is found in the fbc-operons of gram-negative bacteria. In the derived amino acid sequence for the Rieske FeS-protein the four cysteines and the 2 histidines are conserved in the peptides binding the 2Fe2S-cluster, although the redox potential of the cluster is about 150 mV more negative in Chlorobium. The gene for cytochrome b includes the coding region for an N-terminal, positively charged extension which is typical for Chlorobium. The gene is not split into two parts for cytochrome b(6) and subunit IV. However, a fourteenth amino acid between the two histidines in the fourth, putative transmembrane helix, and the lack of an eighth transmembrane helix at the C-terminus, among other features, clearly resemble the cytochrome b(6)f-complexes. Therefore, the separation into b(6)f- and bc(1)-type complexes during evolution must have occurred before the split of the gene.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IRON-SULFUR PROTEIN; CYANOBACTERIUM NOSTOC PCC-7906; NUCLEOTIDE-SEQUENCE; SACCHAROMYCES-CEREVISIAE; BACTERIUM CHLOROBIUM; ELECTRON-TRANSPORT; GENE ORGANIZATION; BC1 COMPLEX; FBC OPERON; DNA; CYTOCHROME BC-COMPLEX; ELECTRON TRANSPORT; GREEN S-BACTERIA; MEMBRANE PROTEINS; PETB; PETC |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53492 |
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