DERMIETZEL, R and HWANG, TK and BUETTNER, R and HOFER, A and DOTZLER, E and KREMER, M and DEUTZMANN, R and THINNES, FP and FISHMAN, GI and SPRAY, DC and SIEMEN, D (1994) CLONING AND IN-SITU LOCALIZATION OF A BRAIN-DERIVED PORIN THAT CONSTITUTES A LARGE-CONDUCTANCE ANION CHANNEL IN ASTROCYTIC PLASMA-MEMBRANES. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 91 (2). pp. 499-503. ISSN 0027-8424,
Full text not available from this repository.Abstract
We have cloned a protein from bovine brain, brain-derived voltage-dependent anion channel 1 (BR1-VDAC), that is identical to a recently sequenced plasmalemmal-bound porin from human lymphocytes. mRNA hybridization indicates that BR1-VDAC is widely distributed throughout nervous and nonnervous tissues. In situ localization substantiated that the BR1-VDAC is associated with the plasmalemma of astrocytes. A monoclonal antibody that recognizes the N terminus of the BR1-VDAC protein completely blocks an astrocytic high-conductance anion channel that has electrophysiological similarities with the mitochondrial VDAC. Since the high-conductance anion channel in astrocytes has been shown to respond to hypoosmotic solutions, its molecular identification provides the basis for a better understanding of volume regulation in brain tissue.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CULTURED RAT ASTROCYTES; GLIAL-CELLS; EXPRESSION; 31HL; ANTIBODIES; PROTEINS; MUSCLE; LINE; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53509 |
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