Mooij, Petra and Koopman, Gerrit and Drijfhout, Jan Wouter and Nieuwenhuis, Ivonne G. and Beenhakker, Niels and Koestler, Josef and Bogers, Willy M. J. M. and Wagner, Ralf and Esteban, Mariano and Pantaleo, Giuseppe and Heeney, Jonathan L. and Jacob, Bertram L. and Melief, Cornelis J. M. (2015) Synthetic long peptide booster immunization in rhesus macaques primed with replication-competent NYVAC-C-KC induces a balanced CD4/CD8 T-cell and antibody response against the conserved regions of HIV-1. JOURNAL OF GENERAL VIROLOGY, 96. pp. 1478-1483. ISSN 0022-1317, 1465-2099
Full text not available from this repository. (Request a copy)Abstract
The Thai trial (RV144) indicates that a prime-boost vaccine combination that induces both T-cell and antibody responses may be desirable for an effective HIV vaccine. We have previously shown that immunization with synthetic long peptides (SLP), covering the conserved parts of SIV, induced strong CD4 T-cell and antibody responses, but only modest CD8 T-cell responses. To generate a more balanced CD4/CD8 T-cell and antibody response, this study evaluated a pox-vector prime/SLP boost strategy in rhesus macaques. Priming with a replication-competent NYVAC, encoding HIV-1 clade C gag, pol and nef, induced modest IFN gamma T-cell immune responses, predominantly directed against HIV-1 Gag. Booster immunization with SLP, covering the conserved parts of HIV-1 Gag, Pol and Env, resulted in a more than 10-fold increase in IFN gamma ELISpot responses in four of six animals, which were predominantly HIV-1 Pol-specific. The animals showed a balanced polyfunctional CD4 and CD8 T-cell response and high Ab titres.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VACCINE; IMMUNOGENICITY; PROTECTION; PROTEIN; ALVAC; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Jul 2019 13:08 |
| Last Modified: | 11 Jul 2019 13:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5376 |
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