HERTER, P and LAUBE, G and GRONCZEWSKI, J and MINUTH, WW (1993) SILVER-ENHANCED COLLOIDAL-GOLD LABELING OF RABBIT KIDNEY COLLECTING-DUCT CELL-SURFACES IMAGED BY SCANNING ELECTRON-MICROSCOPY. JOURNAL OF MICROSCOPY-OXFORD, 171. pp. 107-115. ISSN 0022-2720,
Full text not available from this repository.Abstract
The luminal cell surfaces of rabbit kidney cortical collecting-duct cells were labelled with peanut lectin (PNA) and investigated by scanning electron microscopy. Labelling was performed either on 20-mum-thick cryostat sections from prefixed and cryoprotected rabbit kidney tissue or on cultured collecting-duct epithelium using biotinylated PNA- and a 6-nm colloidal-gold-coupled antibody against biotin. Colloidal-gold labels were detected at low magnification (2000-4000 x) using silver enhancement. Coating with chromium allowed simultaneous imaging of both cell-surface morphology and labelling topography in the backscattered electron imaging mode. Our results show that PNA binding is specific for a subtype of intercalated cells equipped with microvilli on the luminal surface. The presented method promises to be useful for the identification of specific cell types in heterogeneous tissues.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACUTE RESPIRATORY-ACIDOSIS; INTERCALATED CELLS; RAT-KIDNEY; H+-ATPASE; LECTIN; IMMUNOCYTOCHEMISTRY; MICROTUBULES; LOCALIZATION; ANTIBODIES; ANTIGENS; CELL SURFACE; COLLECTING DUCT; IMMUNOGOLD; LECTIN; RABBIT KIDNEY; SCANNING ELECTRON MICROSCOPY; SILVER ENHANCEMENT |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53857 |
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