WAGNER, R and BOLTZ, T and DEML, L and MODROW, S and WOLF, H (1993) INDUCTION OF CYTOLYTIC T-LYMPHOCYTES DIRECTED TOWARDS THE V3 LOOP OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 EXTERNAL GLYCOPROTEIN-GP120 BY P55(GAG)/V3 CHIMERIC VACCINIA VIRUSES. JOURNAL OF GENERAL VIROLOGY, 74. pp. 1261-1269. ISSN 0022-1317,
Full text not available from this repository.Abstract
T cell-mediated cytotoxicity may play an important role in controlling infection by human immunodeficiency virus (HIV). In order to study the ability of rationally designed antigens to induce cytolytic T lymphocytes (CTLs) we replaced stretches of 30 to 50 amino acids at the p17-MA/p24-CA cleavage site, within the p24-CA moiety and within the p6-LI portion of the HIV type 1 p55gag precursor by the third variable domain (V3) of the external glycoprotein gp120. This site is known to be a target for CTL attack in mice and humans. The chimeric antigens were recombined into highly attenuated vaccinia viruses in order to investigate class I major histocompatibility complex (MHC)-restricted presentation of antigenic V3 peptides. Immunoprecipitation and Western blot analysis of the group-specific antigen (p55gag)/V3 chimeric proteins demonstrated significant differences in the accessibility of the V3 domain for a monoclonal antibody or polyclonal V3-specific antisera, depending on the position of the V3 loop within the p55gag carrier protein. Immunization of BALB/c mice with three variants of p55gag/V3 recombinant vaccinia virus, however, resulted in a comparable priming of CD4- CD8+ CTLs in vivo irrelevant of the position of the V3 loop within p55gag. Local conformational changes, including the V3 domain within the p55gag/V3 chimeras, did not demonstrate a significant effect on V3-specific lysis of the target cells when compared to the authentic gp120 envelope protein. Class I MHC-restricted CTLs induced by a V3 consensus sequence cross-reacted perfectly with the LAI strain-derived V3 loop sequence. These data indicate that the combination of selected epitopes (V3) with immunologically relevant complex carrier proteins (p55gag) can be accomplished without the loss of biological activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIV-1 INFECTION INVITRO; MONOCLONAL-ANTIBODIES; ENVELOPE PROTEIN; SYNTHETIC PEPTIDES; SEROPOSITIVE INDIVIDUALS; INFLUENZA HEMAGGLUTININ; CELL RECOGNITION; ACID SEQUENCE; CHIMPANZEES; ANTIGEN; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53900 |
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