BAUMANN, O and TAKEYASU, K (1993) POLARIZED DISTRIBUTION OF NA,K-ATPASE IN HONEYBEE PHOTORECEPTORS IS MAINTAINED BY INTERACTION WITH GLIAL-CELLS. JOURNAL OF CELL SCIENCE, 105. pp. 287-301. ISSN 0021-9533, 1477-9137
Full text not available from this repository.Abstract
Arthropod photoreceptors are polarized cells displaying distinct surface domains. The distribution of the Na,K-ATPase (sodium pump) over these domains was examined in the honeybee photoreceptor using a monoclonal antibody that specifically recognizes the sodium pump alpha-subunit (approximately 100 kDa). We find that the sodium pump is restricted to sites of the nonreceptive photoreceptor surface closely juxtaposed to glial cells; no sodium pumps were detected on the glia-free domains of the nonreceptive surface and on the light-sensitive microvillar membranes. In order to determine the role of photoreceptor-glia contact in maintaining this polarized pump distribution, we assayed the distribution of the Na,K-ATPase after experimentally influencing photoreceptor-glia contact. Sodium pumps were present on the entire nonreceptive photoreceptor surface when photoreceptor-glia contact was removed by isolating the photoreceptors. Remodeling photoreceptor-glia contact by incubation in hyperosmotic saline caused a redistribution of sodium pumps on the photoreceptor surface corresponding to the redistribution of glial cells. We show, further, that both photoreceptor-glia contact and Na,K-ATPase distribution are independent of extracellular Ca2+. No junctional structures were observed at the borders between Na,K-ATPase-positive and Na,K-ATPase-negative membrane domains. Together, these results suggest that adhesion of glial cells to the photoreceptors plays a crucial role in the maintenance of the polarized distribution of Na,K-ATPase in the honeybee photoreceptors. The Ca2+-independent adhesion of glial cells to the photoreceptor surface may trap the pump molecules at the sites of photoreceptor-glia contact.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | KIDNEY EPITHELIAL-CELLS; ADHESION MOLECULE UVOMORULIN; APIS-MELLIFERA MALE; (NA+ + K+)-ATPASE; DROSOPHILA PHOTORECEPTORS; ENDOPLASMIC-RETICULUM; ACTIN-FILAMENTS; COMPOUND EYE; SODIUM-PUMP; IMMUNOCYTOCHEMICAL LOCALIZATION; NA,K-ATPASE; CELL POLARITY; NEURON-GLIA INTERACTION; PHOTORECEPTOR; RETINA; CELL ADHESION MOLECULE |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53940 |
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