HECKMANN, M and ADELMANNGRILL, BC and HEIN, R and KRIEG, T (1993) BIPHASIC EFFECTS OF INTERLEUKIN-1-ALPHA ON DERMAL FIBROBLASTS - ENHANCEMENT OF CHEMOTACTIC RESPONSIVENESS AT LOW CONCENTRATIONS AND OF MESSENGER-RNA EXPRESSION FOR COLLAGENASE AT HIGH-CONCENTRATIONS. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 100 (6). pp. 780-784. ISSN 0022-202X, 1523-1747
Full text not available from this repository.Abstract
Fibrotic reactions in the skin are frequently preceded by infiltration of inflammatory cells and subsequent migration of fibroblastic cells. Interleukin-1 is secreted by inflammatory cells and can regulate proliferation and protein synthesis of fibroblasts. its role in fibroblast chemotaxis has not been elucidated in any detail. Using the well-established Boyden chamber assay for measurement of chemotaxis in vitro, we studied a wide range of recombinant human interleukin-1alpha concentrations to assess intrinsic chemotactic activity of interleukin-1alpha and to determine the capacity of this mediator to modify the chemotactic response of fibroblasts to other chemoattractants. This was compared with the interleukin-1alpha dose required for enhancement of mRNA expression for collagenase. Although interleukin-1alpha was not chemoattractive for fibroblasts, it specifically augmented migration toward fibroblast-conditioned medium and toward platelet-derived growth factor but not toward epidermal growth factor, fibronectin, or transforming-growth factor-beta. Interleukin-1alpha did not measurably alter the expression of mRNA for the platelet-derived growth factor receptor or its platelet-derived growth factor-binding characteristics. Doses required to enhance fibroblast chemotaxis were distinctly lower than those required for stimulation of collagenase mRNA expression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | THYMOCYTE-ACTIVATING FACTOR; GROWTH-FACTOR-BETA; RECOMBINANT INTERLEUKIN-1; INTERFERON-GAMMA; CHEMOATTRACTANTS; MIGRATION; RECEPTOR; ALPHA; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53941 |
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