Sperling, Sergej and Kuehbandner, Stephan and Engel, Katharina C. and Steiger, Sandra and Stoekl, Johannes and Ruther, Joachim (2015) Size Exclusion High Performance Liquid Chromatography: Re-Discovery of a Rapid and Versatile Method for Clean-Up and Fractionation in Chemical Ecology. JOURNAL OF CHEMICAL ECOLOGY, 41 (6). pp. 574-583. ISSN 0098-0331, 1573-1561
Full text not available from this repository. (Request a copy)Abstract
Solvent extraction of bioactive molecules from glands, tissues, or whole organisms is a common first step in chemoecological studies. Co-extraction of a surplus of high boiling materials such as triacylglycerides (TAGs) and other lipids with higher molecular weight might hamper the identification of volatile or medium-volatile semiochemicals by high resolution chromatographic and spectroscopic techniques. Therefore, effective clean-up procedures are needed to separate potential semiochemicals from the accompanying materials. Size exclusion high performance liquid chromatography (SE-HPLC), a technique often disregarded by chemoecologists, has proved to be a rapid and efficient clean-up method for complex crude extracts. We demonstrated that TAGs can be baseline separated from typical semiochemicals within less than 10 min on a porous gel stationary phase based on highly cross-linked polystyrene/divinylbenzene. We applied the method as a rapid one-step clean-up procedure for the analysis of juvenile hormone III in insect hemolymph by gas chromatography-mass spectrometry. We furthermore introduced some recent application examples on insect pheromones to demonstrate that SE-HPLC is not only an effective method for the purification of crude extracts, but can as well be used as a first fractionation step for the bioassay-guided identification of behavior modifying natural products. SE-HPLC can be well operated with low-boiling solvents such as dichloromethane, and results in fraction volumes of typically less than one ml, which decreases the danger of losing volatile analytes during subsequent concentration steps.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FEMALE SEX-PHEROMONE; GEL-PERMEATION CHROMATOGRAPHY; PESTICIDE-RESIDUES; JUVENILE-HORMONE; COLUMN CHROMATOGRAPHY; MULTIRESIDUE ANALYSIS; COURTSHIP BEHAVIOR; BURYING BEETLES; ANIMAL ORIGIN; IDENTIFICATION; Cuticular hydrocarbons; Juvenile hormone; Pheromones; Sample preparation; Triacylglycerides |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Chemische Ökologie (Prof. Dr. Joachim Ruther) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2019 06:14 |
| Last Modified: | 15 Jul 2019 06:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5402 |
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