Zellner, J. and Mueller, M. and Xin, Y. and Krutsch, W. and Brandl, A. and Kujat, R. and Nerlich, M. and Angele, P. (2015) Dynamic hydrostatic pressure enhances differentially the chondrogenesis of meniscal cells from the inner and outer zone. JOURNAL OF BIOMECHANICS, 48 (8). pp. 1479-1484. ISSN 0021-9290, 1873-2380
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This study analyses the influence of dynamic hydrostatic pressure on chondrogenesis of human meniscus-derived fibrochondrocytes and explores the differences in chondrogenic differentiation under loading conditions between cells derived from the avascular inner zone and vascularized outer region of the meniscus. Aggregates of human fibrochondrocytes with cell origin from the inner region or with cell origin from the outer region were generated. From the two groups of either cell origin, aggregates were treated with dynamic hydrostatic pressure (1 Hz for 4 h; 0.55-5.03 MPa, cyclic sinusoidal) from day 1 to day 7. The other aggregates served as unloaded controls. At day 0, 7,14 and 21 aggregates were harvested for evaluation including histology, immunostaining and ELISA analysis for glycosaminoglycan (GAG) and collagen II. Loaded aggregates were found to be macroscopically larger and revealed immunohistochemically enhanced chondrogenesis compared to the corresponding controls. Loaded or non-loaded meniscal cells from the outer zone showed a higher potential and earlier onset of chondrogenesis compared to the cells from the inner part of the meniscus. This study suggests that intrinsic factors like cell properties in the different areas of the meniscus and their reaction on mechanical load might play important roles in designing Tissue Engineering strategies for meniscal repair in vivo. (C) 2015 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL PROGENITOR CELLS; STEM-CELLS; IN-VITRO; MECHANICAL COMPRESSION; GROWTH-FACTORS; KNEE MENISCUS; REPAIR; CULTURE; FIBROCHONDROCYTES; REGENERATION; Meniscus; Fibrochondrocytes; Dynamic hydrostatic pressure; Mechanobiology |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2019 07:10 |
| Last Modified: | 15 Jul 2019 07:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5413 |
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