OPEKAROVA, M and CASPARI, T and TANNER, W (1993) UNIDIRECTIONAL ARGININE TRANSPORT IN RECONSTITUTED PLASMA-MEMBRANE VESICLES FROM YEAST OVEREXPRESSING CAN1. EUROPEAN JOURNAL OF BIOCHEMISTRY, 211 (3). pp. 683-688. ISSN 0014-2956,
Full text not available from this repository.Abstract
Amino acids are accumulated in Saccharomyces cerevisiae by strictly unidirectional influx systems. To see whether cellular compartmentation causes this unusual amino-acid-transport behaviour, arginine transport was studied in plasma-membrane vesicles. The arginine permease gene CAN1 was overexpressed in S. cerevisiae RH218a and in a permease-deficient mutant RS453 (can1). Reconstituted plasma-membrane vesicles from these transformants, energized by incorporated cytochrome-c oxidase, showed 3-4-fold increased rates of arginine uptake compared to vesicles from wild-type cells. The K(T) values were 32.5 muM in vesicles from wild-type and 28.6 muM in vesicles from transformed cells; the corresponding in vivo values were 17.5 muM and 11.4 muM, respectively. It could be demonstrated that unidirectional arginine transport and accumulation also exist in vesicles; thus, unidirectional influx is not related to cellular compartmentation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYTOCHROME-C OXIDASE; PROTON-MOTIVE FORCE; AMINO-ACID-TRANSPORT; SACCHAROMYCES-CEREVISIAE; STREPTOCOCCUS-CREMORIS; GALACTOSE TRANSPORT; GLUCOSE-TRANSPORT; SYSTEMS; PROTEIN; BINDING; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54133 |
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