RUSCHOFF, J and WILLEMER, S and BRUNZEL, M and TRAUTMANN, ME and FRANK, M and ARNOLD, R and KLOPPEL, G (1993) NUCLEOLAR ORGANIZER REGIONS AND GLYCOPROTEIN-HORMONE ALPHA-CHAIN REACTION AS MARKERS OF MALIGNANCY IN ENDOCRINE TUMORS OF THE PANCREAS. HISTOPATHOLOGY, 22 (1). pp. 51-57. ISSN 0309-0167,
Full text not available from this repository.Abstract
The value of silver staining of nucleolar organizer regions (AgNORs) and human chorionic gonadotropin alpha-chain reaction (HCG-alpha) as markers of malignancy was investigated in 60 primary pancreatic endocrine tumours, 37 of which had metastasized at the time of surgery, and in one of which metastases developed 4 years after surgery. Assessment of AgNORs by digital image analysis revealed few but large AgNORs (mean number 2.5 +/- 1.1; mean area 0.32 +/- 0.1 mum2) in the 22 benign tumours and many but small AgNORs (mean number 5.1 +/- 1.9, P < 0.05; mean area 0.18 +/- 0.09 mum2, P < 0.01) in the malignant tumours. Quantification of the number of AgNORs per tumour cell nucleolus (AgNOR distribution score) showed that 96% (26/27) of tumours exhibiting at least 5% of cells with more than six AgNORs per nucleolus showed metastases either at the time of diagnosis or up to 4 years after surgery. HCG-alpha immunoreactive cells were present in 25/38 (66%) malignant tumours and in 4/22 (18%) benign tumours. Combined evaluation of AgNOR distribution and HCG-alpha scores showed a high positive predictive value of 96% in cases with a raised AgNOR distribution score irrespective of the HCG-alpha status. A good negative predictive value (81%) was, however, only obtained if both parameters, AgNOR distribution and HCG-alpha scores, were negative. Thus, investigation of AgNORs and HCG-alpha is helpful in predicting malignancy in a high percentage of pancreatic endocrine tumours.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMAGE-ANALYSIS; TUMORS; PROTEINS; AGNORS; INSULINOMAS; EXPRESSION; PITUITARY; SUBUNITS; ADENOMAS; CELLS; PANCREAS; ENDOCRINE TUMORS; NUCLEOLAR ORGANIZER REGIONS (AGNORS); HUMAN CHORIONIC GONADOTROPIN-(HCG-ALPHA) |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54186 |
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