KLOTH, S and MEYER, D and ROCKL, W and MIETTINEN, A and AIGNER, J and SCHMIDBAUER, A and MINUTH, WW (1992) CHARACTERIZATION OF AN ENDOTHELIAL PROTEIN IN THE DEVELOPING RABBIT KIDNEY. DIFFERENTIATION, 52 (1). pp. 79-88. ISSN 0301-4681,
Full text not available from this repository.Abstract
A new protein (EnPo 1 antigen) abundant on endothelial cells and glomerular podocytes has been characterized by means of the mouse monoclonal antibody EnPo 1. Following electrophoretical separation of rabbit kidney homogenates EnPo 1 recognized a protein with a molecular weight of 110 kDa and an isoelectric point of 5.9 in Western blots. Using immunohistological techniques, the EnPo 1 antigen has been localized in high concentrations on glomerular podocytes of different developmental stages. Furthermore, the EnPo 1 antigen was expressed on endothelial cells of all adult rabbit organs tested so far. Detailed analysis of neonatal rabbit kidney revealed the abundance of EnPo 1 antigen on both differentiated vessels as well as on immature endothelial cells and endothelium of the microvasculature. Thus, for the first time a marker for in situ investigations of angiogenic processes within the mammalian kidney is available. Analysis of kidney cryosections by confocal laser scan microscopy revealed a direct connection between mature and differentiating vessels in the outer kidney cortex. Furthermore, two differentially organized cell populations discriminated by their EnPo 1 binding pattern were localized in the embryonic renal cortex. Morphologically, these cells were not distinguishable from other mesenchymal cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EPITHELIAL-MESENCHYMAL INTERACTIONS; MONOCLONAL-ANTIBODIES; RAT-KIDNEY; CELLS; INDUCTION; ANTIGEN; DIFFERENTIATION; SPECIFICITY; EXPRESSION; MOLECULE; ANGIOGENESIS; ENDOTHELIAL PROTEINS; KIDNEY DEVELOPMENT; MONOCLONAL ANTIBODY; RABBIT |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54263 |
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