KOLLER, G and REEVE, JN and FREY, G and THOMM, M (1992) TRANSCRIPTION INVITRO AND INVIVO OF THE 7S-RNA GENE ASSOCIATED WITH THE RIBOSOMAL-RNA OPERON IN THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS-FERVIDUS. FEMS MICROBIOLOGY LETTERS, 98 (1-3). pp. 95-101. ISSN 0378-1097,
Full text not available from this repository.Abstract
The gene encoding the archaeal 7S RNA in the hyperthermophile Methanothermus fervidus is linked to a tRNA(Ser) and rRNA operon in the arrangement 5'-7S RNA-14nt-tRNA(Ser)-196nt-16S rRNA and the promoter directing transcription of this 7S RNA gene has now been identified. Initiation of transcription of the 7S RNA gene has been shown to occur both in vivo in M. fervidus and in vitro, using a Methanococcus thermolithotrophicus derived cell-free transcription system, at the first G residue within the initiator sequence ATGG, located 6 bp upstream of the 5' end of the 7S RNA coding region. Cotranscription of the 7S RNA and tRNA(Ser) has been demonstrated in vitro.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-FREE TRANSCRIPTION; ESCHERICHIA-COLI; 7S RNA; 4.5S RNA; METHANOCOCCUS-VANNIELII; TATA BOX; ARCHAEBACTERIA; EXPRESSION; METHANOGEN; ELEMENT; METHANOGEN PROMOTER; 7S-RNA; ARCHAEA; THERMOPHILE; METHANOTHERMUS-FERVIDUS |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54308 |
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