Kob, Robert and Fellner, Claudia and Bertsch, Thomas and Wittmann, Astrid and Mishura, Daria and Sieber, Cornel C. and Fischer, Barbara E. and Stroszczynski, Christian and Bollheimer, Cornelius L. (2015) Gender-specific differences in the development of sarcopenia in the rodent model of the ageing high-fat rat. JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE, 6 (2). pp. 181-191. ISSN 2190-5991, 2190-6009
Full text not available from this repository. (Request a copy)Abstract
Background Sarcopenia is linked to functional impairments, loss of independence, and mortality. In the past few years, obesity has been established as being a risk factor for a decline in muscle mass and function. There are several molecular pathological mechanisms, which have been under discussion that might explain this relationship. However, most studies were conducted using male animals and for a short period of time. Methods In this study, the gender-specific effect of long-term, high-fat content feeding in Sprague-Dawley rats was examined. Development of the quadriceps muscle of the animals was monitored in vivo using magnetic resonance. The results of these measurements and of the biochemical analysis of the aged muscle were compared. Results Surprisingly, only male but not female rats showed a decline in muscle cross-sectional area at 16 months of age as a result of a chronic oversupply of dietary fats. This loss of muscle mass could not be explained by either de-regulation of the anabolic Akt pathway or by up-regulation of the main ubiquitin ligases of muscle, MAFbx and MuRF-1. However, fusion of satellite cells to myotubes was induced by the high-fat diet in male rats, possibly as a result of an increased need for compensatory regeneration processes. Caspase-3-dependent apoptosis induction, irrespective of diet, seems to be the major determinant of muscle decline during ageing in male but not female rats. Conclusion Taken together, activation of the apoptosis-inducing Caspase-3 seems to be the most important trigger for the age-related muscle loss. Male rats were more prone to the decline of muscle during ageing than female animals, which was further enforced by a long-term, high fat diet.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SKELETAL-MUSCLE ATROPHY; DIET-INDUCED OBESITY; INSULIN-RESISTANCE; LIPID-ACCUMULATION; PHYSICAL-ACTIVITY; BODY-COMPOSITION; SATELLITE CELLS; ADIPOSE-TISSUE; AGE; APOPTOSIS; Sarcopenic obesity; Lipotoxicity; Ageing skeletal muscle; Metabolism; Gender-specificity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Röntgendiagnostik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2019 08:46 |
| Last Modified: | 15 Jul 2019 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5433 |
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