Nitschke, Wolfgang and Joliot, Pierre and Liebl, Ursula and Rutherford, A. William and Hauska, Günter and Müller, Adolf and Riedel, Astrid (1992) The pH dependence of the redox midpoint potential of the 2Fe2S cluster from cytochrome b6f complex (the ‘Rieske centre’). BIOCHIMICA ET BIOPHYSICA ACTA, 1102 (2). pp. 266-268. ISSN 0006-3002
Full text not available from this repository.Abstract
The pH dependence of the redox midpoint potential (E(m)) of the Rieske centre from spinach cytochrome b6f complex was studied by EPR. The E(m) was found to be independent of pH up to pH 8 and to decrease at higher pH values. The slope of the decrease above the pK value was roughly consistent with the involvement of a dissociable proton on the oxidized form of the cluster. The E(m) in the pH-independent region (i.e., < pH 8) was determined to be + 320 mV. This is in contrast to the original value (E(m) = + 290 mV) reported by Malkin and Aparicio (Biochem. Biophys. Res. Commun. 63 (1975) 1157-1160) and confirms the results reported more recently (E(m) = 310-320 mV) by Malkin (FEBS Lett. 131 (1981) 169-172) and Nitschke et al. (Biochim. Biophys. Acta 974 (1989) 223-226).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELECTRON-TRANSFER; OXIDATION; PROTEIN; CYTOCHROME-B6F COMPLEX; PH DEPENDENCE; IRON-SULFUR CLUSTER; EPR; REDOX POTENTIAL |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Alumni or Retired > Prof. Dr. Günter Hauska Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Petra Gürster |
| Date Deposited: | 07 Nov 2024 09:03 |
| Last Modified: | 07 Nov 2024 09:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54378 |
Actions (login required)
 |
View Item |
