PETERS, J and RUDOLF, S and OSCHKINAT, H and MENGELE, R and SUMPER, M and KELLERMANN, J and LOTTSPEICH, F and BAUMEISTER, W (1992) EVIDENCE FOR TYROSINE-LINKED GLYCOSAMINOGLYCAN IN A BACTERIAL SURFACE PROTEIN. BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 373 (4). pp. 171-176. ISSN 0177-3593,
Full text not available from this repository.Abstract
The S-layer protein of Acetogenium kivui was subjected to proteolysis with different proteases and several high molecular mass glycosaminoglycan peptides containing glucose, galactosamine and an unidentified sugar-related component were separated by molecular sieve chromatography and reversed-phase HPLC and subjected to N-terminal sequence analysis. By methylation analysis glucose was found to be uniformly 1,6-linked, whereas galactosamine was exclusively 1,4-linked. Hydrazinolysis and subsequent amino-acid analysis as well as two-dimensional NMR spectroscopy were used to demonstrate that in these peptides carbohydrate was covalently linked to tyrosine. As all of the four Tyr-glycosylation sites were found to be preceded by valine, a new recognition sequence for glycosylation is suggested.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | S-LAYER PROTEIN; NUCLEOTIDE-SEQUENCE; BIOSYNTHESIS; GLYCOPROTEINS; METHYLATION; PEPTIDE; GLUCOSE; LINKAGE; GENE; GLYCOSAMINOGLYCAN; TYROSINE-BOUND SUGAR; NMR-SPECTROSCOPY; ACETOGENIUM-KIVUI |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54583 |
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