ALLMEIER, H and CRESNAR, B and GRECK, M and SCHMITT, R (1992) COMPLETE NUCLEOTIDE-SEQUENCE OF TN1721 - GENE ORGANIZATION AND A NOVEL GENE-PRODUCT WITH FEATURES OF A CHEMOTAXIS PROTEIN. GENE, 111 (1). pp. 11-20. ISSN 0378-1119, 1879-0038
Full text not available from this repository.Abstract
The complete 11 139-nucleotide sequence of transposon Tn1721 has been determined. It contains three 38-bp inverted repeats, and (in this order) a new orfI, a resolution site (res), genes encoding resolvase (tnpR), transposase (tnpA), tetracycline-resistance (Tc(R)) repressor (tetR), Tc(R) (tetA) and a truncated transposase gene (tnpA'). The modular origin of Tn1721 from at least three separate sources is supported by the distinctive codon usages of orfI, tnpR/tnpA and tetR/tetA, and by sequence similarities with Tn501 (tnpR/tnpA) and RP1 (tetR/tetA). The ORFI-encoded 56-kDa polypeptide exhibits features of a methyl-accepting chemotaxis protein (MCP) with a conserved signal domain and a potential transmembrane domain; this polypeptide cross-reacts with anti-MCP antiserum. Like chemotaxis genes, orfI is transcribed from a sigma-28-like promoter. The overexpressed orfI gene product interferes with MCP-dependent chemotaxis suggesting that it completes for soluble transducer protein(s) in the cell. The potential selective advantage of this novel transposon-borne gene is discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TETRACYCLINE RESISTANCE DETERMINANTS; ESCHERICHIA-COLI; BACTERIAL CHEMOTAXIS; RNA-POLYMERASE; BINDING-SITES; SIGMA-FACTOR; TN501; TN21; TRANSCRIPTION; TRANSDUCERS; TRANSPOSON-BORNE GENES; S1-MAPPING; SIGMA-28-LIKE PROMOTER; CHEMOTAXIS; SIGNAL TRANSDUCTION; TRANSPOSON EVOLUTION |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54656 |
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