Hermann, Fabian J. and Gomez, Manuel Rodriguez and Doser, Kristina and Edinger, Matthias and Hoffmann, Petra and Schiechl, Gabriela and Talke, Yvonne and Goebel, Nicole and Schmidbauer, Kathrin and Syed, Shahzad N. and Bruehl, Hilke and Mack, Matthias (2015) Basophils inhibit proliferation of CD4(+) T cells in autologous and allogeneic mixed lymphocyte reactions and limit disease activity in a murine model of graft versus host disease. IMMUNOLOGY, 145 (2). pp. 202-212. ISSN 0019-2805, 1365-2567
Full text not available from this repository. (Request a copy)Abstract
Basophils are known to modulate the phenotype of CD4(+) T cells and to enhance T helper type 2 responses in vitro and in vivo. In this study, we demonstrate that murine basophils inhibit proliferation of CD4(+) T cells in autologous and allogeneic mixed lymphocyte reactions. The inhibition is independent of Fas and MHC class II, but dependent on activation of basophils with subsequent release of interleukin-4 (IL-4) and IL-6. The inhibitory effect of basophils on T-cell proliferation can be blocked with antibodies against IL-4 and IL-6 and is absent in IL-4/IL-6 double-deficient mice. In addition, we show that basophils and IL-4 have beneficial effects on disease activity in a murine model of acute graft-versus-host disease (GvHD). When basophils were depleted with the antibody MAR-1 before induction of GvHD, weight loss, GvHD score, mortality and plasma tumour necrosis factor levels were increased while injection of IL-4 improved GvHD. Basophil-depleted mice with GvHD also have increased numbers of CD4(+) T cells in the mesenteric lymph nodes. Our data show for the first time that basophils suppress autologous and allogeneic CD4(+) T-cell proliferation in an IL-4-dependent manner.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLLAGEN-INDUCED ARTHRITIS; DENDRITIC CELLS; IL-4 PRODUCTION; MAST-CELLS; IN-VIVO; RESPONSES; ANTIGEN; IMMUNITY; INTERLEUKIN-3; PROMOTE; basophils; CD4(+); T cells; graft-versus-host disease; interleukin-4; interleukin-6; mixed lymphocyte reaction |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Innere Medizin II Medicine > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2019 08:20 |
| Last Modified: | 15 Jul 2019 08:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5468 |
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