KUNKEL, W and BERGER, D and RISCH, S and WITTMANNBRESINSKY, B (1992) GENETIC INSTABILITY OF INDUSTRIAL STRAINS OF PENICILLIUM-CHRYSOGENUM. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 36 (4). pp. 499-502. ISSN 0175-7598,
Full text not available from this repository.Abstract
It has shown that several characteristics of high-producing industrial strains of Penicillium chrysogenum tend to segregate in the course of cultivation (slant-to-slant transfer). Segregation includes a decrease in the yield of penicillin, mean conidial size, mean size of the nuclei, and an increase in the proportion of morphologically wild-type colonies. These lower-producing segregants also have a higher sensitivity against ultraviolet radiation and, as shown by cytofluorometric methods, a lower DNA content in the conidia, a decrease in phosphate uptake and in the activity of extracellular alkaline phosphatases compared to high-producing strains. Obviously, during mutagenesis/selection programmes ploidy mutants have been selected, which entails an increase in the number of genes coding enzymes responsible for penicillin biosynthesis. In the absence of selection pressure these high-producing strains segregate to lower-producing strains by chromosome losses in the course of slant-to-slant transfers.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AMPLIFICATION; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54690 |
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