SPRAY, DC and MORENO, AP and KESSLER, JA and DERMIETZEL, R (1991) CHARACTERIZATION OF GAP-JUNCTIONS BETWEEN CULTURED LEPTOMENINGEAL CELLS. BRAIN RESEARCH, 568 (1-2). pp. 1-14. ISSN 0006-8993,
Full text not available from this repository.Abstract
Leptomeningeal cells in intact meninges or dissociated and cultured for 2 h to several weeks were dye-coupled (Lucifer yellow), and voltage-clamped pairs of freshly dissociated leptomeningeal cells were well coupled electrically. Unitary conductances of junctional channels were predominantly 40-90 pS. Junctional conductance was reversibly reduced by 2 mM halothane, 1 mM heptanol and 100% CO2 and was increased by 1 mM 8 Br-cAMP. Two gap junction proteins, connexin 26 and connexin 43, were identified between leptomeningeal cells using immunocytochemical methods; Northern blot analyses of RNA isolated from cultured leptomeningeal cells showed specific hybridization to cDNAs encoding connexins 26 and 43, but not to a cDNA encoding connexin 32. These studies demonstrate co-expression of two connexins in a single cell type in the nervous system; biophysical properties do not differ significantly from those of astrocytes and cardiac myocytes, which express only connexin 43.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELECTRICAL-PROPERTIES; MOLECULAR-CLONING; RAT-HEART; PROTEIN; LIVER; PHOSPHORYLATION; CONDUCTANCE; HEPATOCYTES; CONNEXIN43; EXPRESSION; CONNEXIN-43; CONNEXIN-26; SINGLE CHANNEL; MESSENGER RIBONUCLEIC ACID; JUNCTIONAL CONDUCTANCE; ELECTRONIC SYNAPSE; HALOTHANE; CYCLIC ADENOSINE MONOPHOSPHATE; MENINGES |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54755 |
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