THE SEXUAL INDUCER OF VOLVOX-CARTERI - ITS LARGE-SCALE PRODUCTION AND SECRETION BY SACCHAROMYCES-CEREVISIAE

HAAS, E and SUMPER, M (1991) THE SEXUAL INDUCER OF VOLVOX-CARTERI - ITS LARGE-SCALE PRODUCTION AND SECRETION BY SACCHAROMYCES-CEREVISIAE. FEBS LETTERS, 294 (3). pp. 282-284. ISSN 0014-5793,

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Abstract

The DNA sequence coding for the sexual inducer glycoprotein of Volvox carteri and its N-terminal signal peptide was placed under the control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a yeast-E. coli shuttle vector. Yeast transformed by this construct synthesized and secreted into the culture medium biologically active inducer in amounts two to three orders of magnitude higher than observed in the Volvox system.

Item Type: Article
Uncontrolled Keywords: F-NAGARIENSIS IYENGAR; PURIFICATION; YEAST; GENE; SEXUAL INDUCER (VOLVOX-CARTERI); HETEROLOGOUS EXPRESSION (SACCHAROMYCES-CEREVISIAE); SECRETION
Depositing User: Dr. Gernot Deinzer
Last Modified: 19 Oct 2022 08:46
URI: https://pred.uni-regensburg.de/id/eprint/54765

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