Directed mutagenesis of the gene psaB of Photosystem I of the cyanobacterium Anabaena variabilis ATCC 29413

Toelge, Martina and Ziegler, Karl and Maldener, Iris and Lockau, Wolfgang (1991) Directed mutagenesis of the gene psaB of Photosystem I of the cyanobacterium Anabaena variabilis ATCC 29413. BIOCHIMICA ET BIOPHYSICA ACTA, 1060 (2). pp. 233-236. ISSN 0006-3002

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Abstract

Two large, hydrophobic polypeptides termed PSI-A and PSI-B of Photosystem I are thought, as a heterodimer, to harbor all redox centers and pigments required for primary charge separation. The gene psaB coding for PSI-B was cloned from Anabaena variabilis, a segment replaced by a drug-resistance cassette, and returned to Anabaena on a suicide plasmid. Double recombinants (mutants) grew only chemoheterotrophically, had a reduced chlorophyll content, and lacked Photosystem I.

Item Type: Article
Uncontrolled Keywords: SYNECHOCYSTIS SP PCC-6803; INSERTIONAL INACTIVATION; CLONING; SELECTION; SUBUNIT; VECTORS; PHOTOSYSTEM-I; GENE-PSAB; PSAB-GENE; DIRECTED MUTAGENESIS; CYANOBACTERIUM; (A-VARIABILIS)
Subjects: 500 Science > 580 Botanical sciences
Divisions: Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften
Depositing User: Petra Gürster
Date Deposited: 07 Nov 2024 09:07
Last Modified: 07 Nov 2024 09:07
URI: https://pred.uni-regensburg.de/id/eprint/54818

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