GALLERT, C and KNOLL, G and WINTER, J (1991) ANAEROBIC CARBOXYLATION OF PHENOL TO BENZOATE - USE OF DEUTERATED PHENOLS REVEALED CARBOXYLATION EXCLUSIVELY IN THE C4-POSITION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 36 (1). pp. 124-129. ISSN 0175-7598,
Full text not available from this repository.Abstract
[U-D]Phenol and [4-D]phenol were used to rule out carboxylation of phenol in the C1-position by a strictly anaerobic, defined mixed culture. By mass spectrometric analysis of deuterated phenol species and of benzoate, which were formed from [U-D]phenol by D/H-exchange or by carboxylation from cell suspensions, it was shown that only one deuterium (D) from the aromatic nucleus was replaced with a least 97% efficiency. This excluded benzoate synthesis by carboxylation in the C1-position of phenol. Finally, carboxylation in the para-position of phenol was demonstrated with [4-D]phenol by gas chromatography/mass spectroscopy of the products. Since direct measurement of phenol carboxylase activity was impossible due to a very active interfering decarboxylase activity, the optimal pH range and ion strength, as well as the requirement of cations in crude cell-free extracts was characterized by means of D/H-exchange from deuterated phenol.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DENITRIFYING PSEUDOMONAS STRAIN; RHODOPSEUDOMONAS-PALUSTRIS; DEGRADATION; 4-HYDROXYBENZOATE; METABOLISM; BACTERIA; COENZYME; PURE; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54824 |
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