BUCHNER, J and RUDOLPH, R (1991) ROUTES TO ACTIVE PROTEINS FROM TRANSFORMED MICROORGANISMS. CURRENT OPINION IN BIOTECHNOLOGY, 2 (4). pp. 532-538. ISSN 0958-1669, 1879-0429
Full text not available from this repository.Abstract
Over-expression of recombinant proteins in microbial hosts results in the formation of active soluble protein or of insoluble aggregates (inclusion bodies). Efficient in vitro refolding strategies have been developed to reactive inactive proteins from inclusion bodies. Co-expression of molecular chaperones may provide a tool to promote correct structure formation of recombinant proteins in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RIBULOSE BISPHOSPHATE CARBOXYLASE; CHEMICALLY SYNTHESIZED GENE; NON-COVALENT AGGREGATION; ESCHERICHIA-COLI; INCLUSION-BODIES; LACTIC-DEHYDROGENASE; BACTERIAL EXPRESSION; RNA-POLYMERASE; BETA-LACTAMASE; ATP HYDROLYSIS; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54931 |
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