CHEONG, GW and CEJKA, Z and PETERS, J and STETTER, KO and BAUMEISTER, W (1991) THE SURFACE PROTEIN LAYER OF METHANOPLANUS-LIMICOLA - 3-DIMENSIONAL STRUCTURE AND CHEMICAL CHARACTERIZATION. SYSTEMATIC AND APPLIED MICROBIOLOGY, 14 (3). pp. 209-217. ISSN 0723-2020,
Full text not available from this repository.Abstract
Methanoplanus limocola, a member of a novel archaebacterial family, the Methanoplanaceae, possesses a hexagonally arrayed surface glycoprotein (S-layer). We have determined the molecular architecture of this surface glycoprotein via three-dimensional reconstruction from a tilt series of negatively stained preparations and surface relief reconstructions of metal shadowed preparations. The surface layer has p6 symmetry, a lattice constant of 14.7 nm and thickness of approx. 4.5 nm. The S-layer protein has an apparent molecular weight of 135 kDa as determined by SDS-polyacrylamide gel electrophoresis, but this value shifts to 115 kDa after treatment with anhydrous trifluoromethanesulfonic acid. This observation, together with quantitative estimations revealing a total neutral sugar content of 240 mg/g polypeptide suggests that the S-layer protein of Methanoplanus limicola is a glycoprotein.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEOTIDE-SEQUENCE; GENE; RECONSTRUCTION; GLYCOPROTEINS; CLONING; SYSTEM; ACID; ARCHAEBACTERIA; METHANOPLANUS-LIMICOLA; BACTERIAL SURFACE LAYER; GLYCOPROTEIN; 3-DIMENSIONAL RECONSTRUCTION; DEGLYCOSYLATION |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/54976 |
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