KLITSCH, T and SIEMEN, D (1991) INNER MITOCHONDRIAL-MEMBRANE ANION CHANNEL IS PRESENT IN BROWN ADIPOCYTES BUT IS NOT IDENTICAL WITH THE UNCOUPLING PROTEIN. JOURNAL OF MEMBRANE BIOLOGY, 122 (1). pp. 69-75. ISSN 0022-2631,
Full text not available from this repository.Abstract
Vesicles of inner mitochondrial membrane, mitoplasts, from rat brown adipose tissue were prepared by osmotic swelling and studied using the patch-clamp technique. Current events of a 107.8 +/- 8.7 pS (n = 16, 21-degrees-C) channel were recorded in the mitoplast-attached mode. This channel was selective for anions and its kinetics resembled those of channels previously found in liver and heart mitochondria of mouse and ox. In whole-mitoplast mode each of five purine nucleotides (20-mu-M) blocked the channel. This is the first demonstration of pharmacological blockade of this type of channel. Although a similar anion channel in mouse and ox mitochondria was suggested to be the uncoupling protein (UCP) associated with nonshivering thermogenesis, we present several arguments against this possibility. Thus we describe a high-conductance, purine-nucleotide-binding, anion selective mitochondrial channel, that is not the UCP.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ADIPOSE-TISSUE MITOCHONDRIA; FAT MITOCHONDRIA; ION CHANNEL; PERMEABILITY; NUCLEOTIDES; BINDING; PROTON; INNER MITOCHONDRIAL MEMBRANE; ANION CHANNEL; THERMOGENIN; UNCOUPLING PROTEIN; PURINE NUCLEOTIDES; GUANOSINE 5'-DIPHOSPHATE; BROWN ADIPOSE TISSUE |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/55015 |
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