BERNGES, F and HOLLER, E (1991) THE REACTION OF PLATINUM(II) COMPLEXES WITH DNA - KINETICS OF INTRASTRAND CROSS-LINK FORMATION INVITRO. NUCLEIC ACIDS RESEARCH, 19 (7). pp. 1483-1489. ISSN 0305-1048,
Full text not available from this repository.Abstract
The kinetics of the formation of bifunctional DNA platinum(II) adducts (DNA-crosslinks) have been investigated by endonuclease digestion and subsequent HPLC analysis of the soluble nucleotides and nucleotide platinum(II) adducts. The results indicate two waves of crosslinking [rate constants (0.2 - 0.3) min-1 and (0.015 - 0.025) min-1] that correlate with changes in ultra violet absorbance and ethidium bromide dependent fluorescence intensity, previously interpreted in terms of two consecutive, local conformational rearrangements of platinum-DNA (Schaller, W., Reisner, H., and Holler, E. (1987) Biochemistry 26, 943-950). The formation of crosslinks at sequences d(GpG) and d(GpNpG) follows identical kinetics. A minimal reaction mechanism is proposed for the binding of cis-diamminedichloro-platinum(II) to DNA under in vitro conditions. The approximately 3-fold higher rate for meso-[1,2-bis(2,6-dichloro-4-hydroxyphenyl)ethylenediamine]diaquaplatinum(II) in comparison to the rate for cis-diammine-diaquaplatinum(II) indicates that crosslink formation is affected by the nature of the non-leaving platinum ligand(s).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ANTITUMOR DRUG CIS-DIAMMINEDICHLOROPLATINUM(II); MECHANISM; ADDUCTS; BINDING; CIS-DICHLORO(ETHYLENEDIAMINE)PLATINUM(II); CONFORMATION; SEQUENCE; REGIONS; MODEL; ACID; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/55026 |
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