Berner, Arne and Henkel, Jan and Woodruff, Maria A. and Steck, Roland and Nerlich, Michael and Schuetz, Michael A. and Hutmacher, Dietmar W. (2015) Delayed Minimally Invasive Injection of Allogenic Bone Marrow Stromal Cell Sheets Regenerates Large Bone Defects in an Ovine Preclinical Animal Model. STEM CELLS TRANSLATIONAL MEDICINE, 4 (5). pp. 503-512. ISSN 2157-6564, 2157-6580
Full text not available from this repository. (Request a copy)Abstract
Cell-based tissue engineering approaches are promising strategies in the field of regenerative medicine. However, the mode of cell delivery is still a concern and needs to be significantly improved. Scaffolds and/or matrices loaded with cells are often transplanted into a bone defect immediately after the defect has been created. At this point, the nutrient and oxygen supply is low and the inflammatory cascade is incited, thus creating a highly unfavorable microenvironment for transplanted cells to survive and participate in the regeneration process. We therefore developed a unique treatment concept using the delayed injection of allogenic bone marrow stromal cell (BMSC) sheets to regenerate a critical-sized tibial defect in sheep to study the effect of the cells' regeneration potential when introduced at a postinflammatory stage. Minimally invasive percutaneous injection of allogenic BMSCs into biodegradable composite scaffolds 4 weeks after the defect surgery led to significantly improved bone regeneration compared with preseeded scaffold/cell constructs and scaffold-only groups. Biomechanical testing and microcomputed tomography showed comparable results to the clinical reference standard (i.e., an autologous bone graft). To our knowledge, we are the first to show in a validated preclinical large animal model that delayed allogenic cell transplantation can provide applicable clinical treatment alternatives for challenging bone defects in the future.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; MEDICINE; THERAPY; REPAIR; RECONSTRUCTION; PROGENITOR; STRATEGIES; SKELETAL; DELIVERY; Large bone defect; Mesenchymal stem cells; Allogenic; Bone tissue engineering; Cell injection; Bone regeneration; Sheep |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Jul 2019 11:19 |
| Last Modified: | 18 Jul 2019 11:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5552 |
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