Wurster, Eva-Christina and Liebl, Renate and Michaelis, Stefanie and Robelek, Rudolf and Wastl, Daniel S. and Giessibl, Franz J. and Goepferich, Achim and Breunig, Miriam (2015) Oligo layer-Coated Nanoparticles: Impact of Surface Topography at the Nanobio Interface. ACS APPLIED MATERIALS & INTERFACES, 7 (15). pp. 7891-7900. ISSN 1944-8244,
Full text not available from this repository. (Request a copy)Abstract
Layer-by-layer coating of nanoparticles with a layer number in the single-digit range has gained increasing attention in the field of nanomedicinal research. However, the impact of using various polyelectrolytes on oligolayer formation and, more importantly, their influence on the interaction with the biological system has not often been considered in the past. Hence, we investigated the polyelectrolyte deposition profiles and resulting surface topographies of up to three polyelectrolyte layers on a flat gold sensor surface using three different polycations, namely, poly(ethylene imine) (PEI), poly(allylamine hydrochloride) (PAR), and poly(diallylammonium chloride) (PD), each in combination with poly(styrenesulfonate) (PSS). Surface plasmon resonance spectroscopy and atomic force Microscopy revealed that the PEI/PSS pair in particular showed a so-called overshoot phenomenon, which is associated with partial polyelectrolyte desorption from the surface. This is also reflected by a significant increase in the surface roughness. Then, after having transferred the oligolayer assembly onto nanoparticles of similar to 32nm, we realized that quite similar surface topographies must have emerged on a curved gold surface. A major finding was that the extent of surface roughness contributes significantly to the fashion by which the oligolayer-coated nanoparticles interact with serum proteins and associate with cells. For example, for the PEI/PSS system, both the surface roughness and protein adsorption increased by a factor of similar to 12 from the second to third coating layer and, at the same time, the cell association massively decreased to only one-third. Our study shows that surface roughness, along with other particle properties such as size, shape, zeta potential, and hydrophobicity, is another decisive factor for nanoparticles in a biological context, which has indeed been discussed previously but has not to date been investigated for oligolayers.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ATOMIC-FORCE MICROSCOPY; FUNCTIONAL CORE/SHELL NANOPARTICLES; GLASSY-CARBON ELECTRODES; POLYELECTROLYTE MULTILAYERS; GOLD NANOPARTICLES; PROTEIN ADSORPTION; SIRNA DELIVERY; WEAK POLYELECTROLYTES; FILM ELECTRODES; CHARGE-REVERSAL; Layer-by-layer; planar surface; AFM; nanoparticle; protein adsorption; cell association |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jul 2019 12:53 |
| Last Modified: | 22 Jul 2019 12:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5627 |
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