Vogler, Frank and Konrad, Sebastian S. A. and Sprunck, Stefanie (2015) Knockin' on pollen's door: live cell imaging of early polarization events in germinating Arabidopsis pollen. FRONTIERS IN PLANT SCIENCE, 6: 246. ISSN 1664-462X,
Full text not available from this repository. (Request a copy)Abstract
Pollen tubes are an excellent system for studying the cellular dynamics and complex signaling pathways that coordinate polarized tip growth. Although several signaling mechanisms acting in the tip-growing pollen tube have been described, our knowledge on the subcellular and molecular events during pollen germination and growth site selection at the pollen plasma membrane is rather scarce. To simultaneously track germinating pollen from up to 12 genetically different plants we developed an inexpensive and easy mounting technique, suitable for every standard microscope setup. We performed high magnification live-cell imaging during Arabidopsis pollen activation, germination, and the establishment of pollen tube tip growth by using fluorescent marker lines labeling either the pollen cytoplasm, vesicles, the actin cytoskeleton or the sperm cell nuclei and membranes. Our studies revealed distinctive vesicle and F-actin polarization during pollen activation and characteristic growth kinetics during pollen germination and pollen tube formation. Initially, the germinating Arabidopsis pollen tube grows slowly and forms a uniform roundish bulge, followed by a transition phase with vesicles heavily accumulating at the growth site before switching to rapid tip growth. Furthermore, we found the two sperm cells to be transported into the pollen tube after the phase of rapid tip growth has been initiated. The method presented here is suitable to quantitatively study subcellular events during Arabidopsis pollen germination and growth, and for the detailed analysis of pollen mutants with respect to pollen polarization, bulging, or growth site selection at the pollen plasma membrane.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ROOT HAIR DEVELOPMENT; TUBE TIP GROWTH; ACTIN CYTOSKELETON; GENETIC-CONTROL; MEMBRANE TRAFFICKING; PLANT-CELL; ORGANIZATION; DYNAMICS; ACTIVATION; MECHANISMS; pollen activation; cell polarization; bulging; vesicular trafficking; ARO1; actin cytoskeleton; sperm cells |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jul 2019 12:13 |
| Last Modified: | 22 Jul 2019 12:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5628 |
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