Rothenhoefer, Martin and Grundmann, Marco and Bernhardt, Guenther and Matysik, Frank-Michael and Buschauer, Armin (2015) High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) for the sensitive determination of hyaluronan oligosaccharides. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 988. pp. 106-115. ISSN 1570-0232, 1873-376X
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High performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) was optimized for the analysis of oligosaccharides derived from the extracellular matrix component hyaluronan. Using this sensitive approach, the separation of oligosaccharides consisting of two (molecular weight ca. 0.8 kDa) up to 25-30 (molecular weight: ca. 9.5-11.4kDa) disaccharide moieties was possible. Standard oligosaccharides (comprising 2-4 repetitive disaccharides) were detectable at very low amounts of 0.2-0.3 pmol (20-30 nM). Including 10 min of column equilibration, a complex mixture of low molecular weight hyaluronan can be analyzed within 40 min. The HPAEC method was successfully applied to the study of the size-dependency of both the action of bovine testicular hyaluronidase (BTH) and the precipitation of hyaluronan by cetyltrimethylammonium bromide (CTAB), a physicochemical reaction often used for the determination of hyaluronan and hyaluronidase activity. (C) 2015 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BOVINE TESTICULAR HYALURONIDASE; POLYACRYLAMIDE-GEL ELECTROPHORESIS; IONIZATION MASS-SPECTROMETRY; BETA-GALACTOSIDASE; CANCER BIOLOGY; ACID; MECHANISM; PRODUCTS; GLYCOSAMINOGLYCANS; QUANTITATION; Bovine testicular hyaluronidase; Capillary electrophoresis; High performance anion exchange chromatography; Hyaluronan oligosaccharides; Pulsed amperometric detection; Turbidimetric hyaluronidase assay |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jul 2019 12:22 |
| Last Modified: | 22 Jul 2019 12:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5636 |
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