Eckl, Daniel B. and Landgraf, Nicole and Hoffmann, Anja K. and Eichner, Anja and Huber, Harald and Baeumler, Wolfgang (2023) Photodynamic Inactivation of Bacteria in Ionic Environments Using the Photosensitizer SAPYR and the Chelator Citrate. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 99 (2). pp. 716-731. ISSN 0031-8655, 1751-1097
Full text not available from this repository. (Request a copy)Abstract
Many studies show that photodynamic inactivation (PDI) is a powerful tool for the fight against pathogenic, multiresistant bacteria and the closing of hygiene gaps. However, PDI studies have been frequently performed under standardized in vitro conditions comprising artificial laboratory settings. Under real-life conditions, however, PDI encounters substances like ions, proteins, amino acids and fatty acids, potentially hampering the efficacy of PDI to an unpredictable extent. Thus, we investigated PDI with the phenalene-1-one-based photosensitizer SAPYR against Escherichia coli and Staphylococcus aureus in the presence of calcium or magnesium ions, which are ubiquitous in potential fields of PDI applications like in tap water or on tissue surfaces. The addition of citrate should elucidate the potential as a chelator. The results indicate that PDI is clearly affected by such ubiquitous ions depending on its concentration and the type of bacteria. The application of citrate enhanced PDI, especially for Gram-negative bacteria at certain ionic concentrations (e.g. CaCl2 or MgCl2: 7.5 to 75 mmol L-1). Citrate also improved PDI efficacy in tap water (especially for Gram-negative bacteria) and synthetic sweat solution (especially for Gram-positive bacteria). In conclusion, the use of chelating agents like citrate may facilitate the application of PDI under real-life conditions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DRINKING-WATER; ANTIMICROBIAL ACTIVITY; DIVALENT-CATIONS; SINGLET OXYGEN; STAPHYLOCOCCUS-AUREUS; SWEAT COMPOSITION; TAP WATER; IN-VITRO; MAGNESIUM; PHOTOINACTIVATION |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Feb 2024 09:37 |
| Last Modified: | 20 Feb 2024 09:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56444 |
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