Kneuttinger, Andrea C. (2022) A guide to designing photocontrol in proteins: methods, strategies and applications. BIOLOGICAL CHEMISTRY, 403 (5-6). pp. 573-613. ISSN 1431-6730, 1437-4315
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Light is essential for various biochemical processes in all domains of life. In its presence certain proteins inside a cell are excited, which either stimulates or inhibits subsequent cellular processes. The artificial photocontrol of specifically proteins is of growing interest for the investigation of scientific questions on the organismal, cellular and molecular level as well as for the development of medicinal drugs or biocatalytic tools. For the targeted design of photocontrol in proteins, three major methods have been developed over the last decades, which employ either chemical engineering of small-molecule photosensitive effectors (photopharmacology), incorporation of photoactive non-canonical amino acids by genetic code expansion (photoxenoprotein engineering), or fusion with photoreactive biological modules (hybrid protein optogenetics). This review compares the different methods as well as their strategies and current applications for the light-regulation of proteins and provides background information useful for the implementation of each technique.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENETIC-CODE EXPANSION; SITE-SPECIFIC INCORPORATION; TRANSFER-RNA-SYNTHETASES; AMINO-ACID MUTAGENESIS; OPTICAL CONTROL; LIGHT-ACTIVATION; BIOLOGICAL-ACTIVITY; OPTOGENETIC CONTROL; SPATIOTEMPORAL CONTROL; INTERNAL ILLUMINATION; light-regulation; non-canonical amino acids; optogenetics; photopharmacology; photoxenoprotein engineering; proteins |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner |
| Depositing User: | Petra Gürster |
| Date Deposited: | 01 Sep 2023 07:02 |
| Last Modified: | 29 Feb 2024 14:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56451 |
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