Gluck, Vivian and Tydykov, Leonid and Mader, Anna-Lena and Warda, Anne-Sophie and Bertok, Manuela and Weidlich, Tanja and Gottwald, Christine and Kostler, Josef and Salzberger, Bernd and Wagner, Ralf and Koller, Michael and Gessner, Andre and Schmidt, Barbara and Glueck, Thomas and Peterhoff, David (2022) Humoral immunity in dually vaccinated SARS-CoV-2-naive individuals and in booster-vaccinated COVID-19-convalescent subjects. INFECTION, 50 (6). pp. 1475-1481. ISSN 0300-8126, 1439-0973
Full text not available from this repository. (Request a copy)Abstract
Background The immune response to COVID-19-vaccination differs between naive vaccinees and those who were previously infected with SARS-CoV-2. Longitudinal quantitative and qualitative serological differences in these two distinct immunological subgroups in response to vaccination are currently not well studied. Methods We investigate a cohort of SARS-CoV-2-naive and COVID-19-convalescent individuals immediately after vaccination and 6 months later. We use different enzyme-linked immunosorbent assay (ELISA) variants and a surrogate virus neutralization test (sVNT) to measure IgG serum titers, IgA serum reactivity, IgG serum avidity and neutralization capacity by ACE2 receptor competition. Results Anti-receptor-binding domain (RBD) antibody titers decline over time in dually vaccinated COVID-19 naives whereas titers in single dose vaccinated COVID-19 convalescents are higher and more durable. Similarly, antibody avidity is considerably higher among boosted COVID-19 convalescent subjects as compared to dually vaccinated COVID-19-naive subjects. Furthermore, sera from boosted convalescents inhibited the binding of spike-protein to ACE2 more efficiently than sera from dually vaccinated COVID-19-naive subjects. Conclusions Long-term humoral immunity differs substantially between dually vaccinated SARS-CoV-2-naive and COVID-19-convalescent individuals. Booster vaccination after COVID-19 induces a more durable humoral immune response in terms of magnitude and quality as compared to two-dose vaccination in a SARS-CoV-2-naive background.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COVID-19; ANTIBODY; COVID-19; SARS-CoV-2; Antibody-mediated immunity; SARS-CoV-2-vaccination; Avidity; Surrogate neutralization |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Klinische Studien Medicine > Abteilung für Krankenhaushygiene und Infektiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Oct 2023 14:40 |
| Last Modified: | 17 Oct 2023 14:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56505 |
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