Stahl, Monika and Holzinger, Jonas and Buelow, Sigrid and Goepferich, Achim M. (2022) Enzyme-triggered antigen release enhances cross-presentation by dendritic cells. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 42: 102545. ISSN 1549-9634, 1549-9642
Full text not available from this repository. (Request a copy)Abstract
Nanoparticles hold great potential as vaccine carriers due to their highly versatile structure and the possibility to influence intracellular trafficking and antigen presentation by their design. In this study, we developed a nanoparticulate system with a new enzyme-triggered antigen release mechanism. For this novel approach, nanoparticle and model antigen ovalbumin were linked with a substrate of the early endosomal protease cathepsin S. This construct enabled the transfer of antigens delivered to bone marrow-derived dendritic cells from the endo-lysosomal compartments in the cytosol. Consecutively, our particles enhanced cross-presentation on dendritic cells and subsequently promoted a stronger activation of CD8+ T cells. Our findings suggest that enzyme-triggered antigen release allows the endosomal escape of the antigen, leading to increased MHC-I presentation. Since T cell immunity is central for the control of viral infections and cancer, this release mechanism offers a promising approach for the development of both prophylactic and therapeutic vaccines. (c) 2022 Published by Elsevier Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VACCINES; DELIVERY; NANOPARTICLES; OVALBUMIN; PEPTIDES; IMMUNITY; RECEPTOR; BIOLOGY; SIZE; Nanoparticles; Cross-presentation; Ovalbumin; Cathepsin S; Enzymatic |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Oct 2023 15:09 |
| Last Modified: | 31 Oct 2023 15:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56510 |
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