Interim Storage of Biospecimen at Satellite Collection Centers: Dewar and Cryotube Choice Are Important for Temporary Storage in Liquid Nitrogen

Niedermair, Tanja and Bhatt, Meet and Babel, Maximilian and Feustel, Moritz and Mamilos, Andreas and Schweikl, Helmut and Ferstl, Gerlinde and Hofman, Paul and Brochhausen, Christoph (2023) Interim Storage of Biospecimen at Satellite Collection Centers: Dewar and Cryotube Choice Are Important for Temporary Storage in Liquid Nitrogen. BIOPRESERVATION AND BIOBANKING, 21 (2). pp. 149-157. ISSN 1947-5535, 1947-5543

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Abstract

One major goal of biobanks is to provide the best possible biospecimen quality for research use. This can be achieved, notably in accredited structures, by using standardized procedures for collection, processing, and storage of biosamples and associated data. Since tissue samples of a clinical biobank are commonly collected at surgical theaters in satellite locations or hospitals in remote areas, adequate temporary storage of the biosample is mandatory to maintain optimal sample quality. In cases where immediate snap freezing of the collected material is possible, interim storage of the samples in portable dewars filled with liquid nitrogen (LN2) is a widely used method. Therefore, the ideal dewar size and maximum storage time need to be considered to maintain an optimal biospecimen quality. In addition, the nature of the cryotube material is an important aspect for keeping the biosample safe while storing it in LN2. The objective of this study was to test different dewar vessels with respect to LN2 volume and consumption and to analyze the impact of LN2 contact on cryotube material through scanning electron microscopy.

Item Type: Article
Uncontrolled Keywords: biobank; biospecimen; biosample; quality; cryotube; liquid nitrogen
Subjects: 500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Pathologie
Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Prof. Dr. rer. nat. Helmut Schweikl
Depositing User: Petra Gürster
Date Deposited: 10 Sep 2024 07:01
Last Modified: 10 Sep 2024 07:01
URI: https://pred.uni-regensburg.de/id/eprint/56607

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