Steines, Louisa and Scharf, Mona and Hoffmann, Petra and Schuster, Antonia and Banas, Bernhard and Bergler, Tobias (2022) Monitoring B cell alloresponses in rats. JOURNAL OF IMMUNOLOGICAL METHODS, 501: 113212. ISSN 0022-1759, 1872-7905
Full text not available from this repository. (Request a copy)Abstract
Antibody-mediated rejection is a major cause of graft failure in organ transplantation. For this reason, B cell responses are of particular interest to transplantation research. Rats are important model organisms for transplant studies, but B cell alloimmune assays and B cell subset markers are poorly established in rats. We alloimmunized rats by donor blood injection using the high responder rat strain combination Brown Norway (donor) and Lewis (recipient) rats. Using splenocytes from alloimmunized and control rats, we established assays to assess allospecific B cell proliferation and the capacity to generate allospecific B memory cells and alloantibody-secreting cells after antigenic rechallenge in vitro using a mixed lymphocyte reaction. Furthermore, we defined a simple gating and sorting strategy for pre-and post-germinal center follicular B cells, as well as non switched and switched plasmablasts. Our protocols for assessing B cell alloresponses and B cell subsets in rats may help to accelerate research into the role of B cells and manipulation of humoral alloresponses in transplant research.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHRONIC REJECTION; TOLERANCE; TRANSPLANTATION; ANTIBODIES; BREAKDOWN; MODELS; Kidney transplantation; B cells; Rat model; Donor-specific antibodies; Alloresponses |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Abteilung für Nephrologie Medicine > Zentren des Universitätsklinikums Regensburg > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jan 2024 06:10 |
| Last Modified: | 26 Jan 2024 06:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56629 |
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