Mao, Xiaojun and Hiergeist, Andreas and Auer, David L. and Scholz, Konstantin J. and Muehler, Denise and Hiller, Karl-Anton and Maisch, Tim and Buchalla, Wolfgang and Hellwig, Elmar and Gessner, Andre and Al-Ahmad, Ali and Cieplik, Fabian (2022) Ecological Effects of Daily Antiseptic Treatment on Microbial Composition of Saliva-Grown Microcosm Biofilms and Selection of Resistant Phenotypes. FRONTIERS IN MICROBIOLOGY, 13: 934525. ISSN , 1664-302X
Full text not available from this repository. (Request a copy)Abstract
Antiseptics are widely used in dental practice and included in numerous over-the-counter oral care products. However, the effects of routine antiseptic use on microbial composition of oral biofilms and on the emergence of resistant phenotypes remain unclear. Microcosm biofilms were inoculated from saliva samples of four donors and cultured in the Amsterdam Active Attachment biofilm model for 3 days. Then, they were treated two times daily with chlorhexidine digluconate (CHX) or cetylpyridinium chloride (CPC) for a period of 7 days. Ecological changes upon these multiple antiseptic treatments were evaluated by semiconductor-based sequencing of bacterial 16S rRNA genes and identification of amplicon sequence variants (ASVs). Furthermore, culture-based approaches were used for colony-forming units (CFU) assay, identification of antiseptic-resistant phenotypes using an agar dilution method, and evaluation of their antibiotic susceptibilities. Both CHX and CPC showed only slight effects on CFU and could not inhibit biofilm growth despite the two times daily treatment for 7 days. Both antiseptics showed significant ecological effects on the microbial compositions of the surviving microbiota, whereby CHX led to enrichment of rather caries-associated saccharolytic taxa and CPC led to enrichment of rather gingivitis-associated proteolytic taxa. Antiseptic-resistant phenotypes were isolated on antiseptic-containing agar plates, which also exhibited phenotypic resistance to various antibiotics. Our results highlight the need for further research into potential detrimental effects of antiseptics on the microbial composition of oral biofilms and on the spread of antimicrobial resistance in the context of their frequent use in oral healthcare.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STREPTOCOCCUS-MUTANS; GLIDING BACTERIA; IN-VITRO; CHLORHEXIDINE; MOUTHWASHES; chlorhexidine; cetylpyridinium chloride; antiseptic; biofilm; resistance; biocide; antibiotic; dysbiosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Feb 2024 08:11 |
| Last Modified: | 13 Feb 2024 08:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56632 |
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