Fadil, Fadi and Samol, Claudia and Berger, Raffaela S. and Kellermeier, Fabian and Gronwald, Wolfram and Oefner, Peter J. and Dettmer, Katja (2022) Isotope Ratio Outlier Analysis (IROA) for HPLC-TOFMS-Based Metabolomics of Human Urine. METABOLITES, 12 (8): 741. ISSN , 2218-1989
Full text not available from this repository. (Request a copy)Abstract
Metabolic fingerprinting by mass spectrometry aims at the comprehensive, semiquantitative analysis of metabolites. Isotope dilution, if successfully implemented, may provide a more reliable, relative quantification. Therefore, the C-13 labeled yeast extract of the IROA TruQuant kit was added as an internal standard (IS) to human urine samples measured in full-scan mode on a high-performance liquid chromatography-time-of-flight mass spectrometer (HPLC-TOFMS) system. The isotope ratio approach enabled the analysis of 112 metabolites. The correlation with reference data did not improve significantly using C-12/C-13 ratios compared to absolute C-12 peak areas. Moreover, using an intricate C-13-labeled standard increased the complexity of the mass spectra, which made correct signal annotation more challenging. On the positive side, the ratio approach helps to reduce batch effects, but it does not perform better than computational methods such as the "removebatcheffect" function in the R package Limma.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MASS-SPECTROMETRY; QUANTIFICATION; DILUTION; NORMALIZATION; METABOLITES; ANNOTATION; ABSOLUTE; metabolomics; metabolic fingerprinting; stable isotope; HPLC; mass spectrometry; labeled yeast extract; IROA; relative quantification |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Nov 2023 09:51 |
| Last Modified: | 07 Nov 2023 09:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56659 |
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