Meister, Toni Luise and Gottsauner, Josef-Maximilian and Schmidt, Barbara and Heinen, Natalie and Todt, Daniel and Audebert, Franz and Buder, Felix and Lang, Henriette and Gessner, Andre and Steinmann, Eike and Vielsmeier, Veronika and Pfaender, Stephanie and Cieplik, Fabian (2022) Mouthrinses against SARS-CoV-2-High antiviral effectivity by membrane disruption in vitro translates to mild effects in a randomized placebo-controlled clinical trial. VIRUS RESEARCH, 316: 198791. ISSN 0168-1702, 1872-7492
Full text not available from this repository. (Request a copy)Abstract
The emergence of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) represents an unprecedented threat for the human population, necessitating rapid and effective intervention measures. Given the main infection route by airborne transmission, significant attention has been bestowed upon the use of antiseptic mouthrinses as a way to possibly reduce infectious viral titers. However, clinical evaluations are still sparse. Thus, we evaluated a wide variety of antiseptic agents that can be used as mouthrinses for their antiviral effects in vitro and their respective mode of action. One of the most promising antiseptic agents (benzalkoniumchloride, BAC) was used in a randomized placebo-controlled clinical trial with subsequent analysis of viral loads by RT-qPCR and virus rescue in cell culture. Mechanistic analysis revealed that treatment with BAC and other antiseptic agents efficiently inactivated SARS-CoV-2 in vitro by primarily disrupting the viral envelope, without affecting viral RNA integrity. However, the clinical application only resulted in a mild reduction of viral loads in the oral cavity. These results indicate that gargling with mouthrinses comprising single antiseptic agents may play a minor role towards a potential reduction of transmission rates and thus, these findings are of utmost importance when considering alternative COVID-19 prevention strategies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EFFICACY; SARS-CoV-2; Antiseptic agents; Mouthrinse; Benzalkonium chloride; Capsid protection assay; Mouthwash |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Zahnärztliche Prothetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Nov 2023 10:23 |
| Last Modified: | 07 Nov 2023 10:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56673 |
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