Berber, Patricia and Bondarenko, Sofiia and Michaelis, Lisa and Weber, Bernhard Heinrich Friedrich (2022) Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (23): 14893. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Retinal organoids (ROs) are three-dimensional retinal tissues, which are differentiated in vitro from induced pluripotent stem cells (iPSC), ultimately forming all main retinal cell types under defined culture conditions. ROs show several highly specialized retinal features, including the outgrowth of photoreceptor outer segments (OSs). In vivo, the photoreceptor OSs are enveloped and maintained by protrusions of retinal pigment epithelium (RPE) cells, the so-called apical microvilli, while ROs fail to recapitulate this critical interaction in culture development. Here, we define specific co-culture conditions aiming to compensate for the missing physical proximity of RPE and OSs in RO development. Accordingly, functional RPE cells and ROs were differentiated simultaneously from the same iPSC clone, the former resulting in byproduct RPE or bRPE cells. While some co-culture approaches indicated a temporary functional interaction between bRPE and RO photoreceptors, they did not improve the photoreceptor histoarchitecture. In contrast, embedding ROs in a basement membrane extract without bRPE cells showed a robust improvement in the rate of photoreceptor OS retention. RO embedding is a quick and easy method that greatly enhances the preservation of photoreceptor OSs, an important structure for modelling retinal diseases with the involvement of photoreceptors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN FETAL; STEM-CELLS; induced pluripotent stem cells; retinal organoids; photoreceptors; outer segments; retinal pigment epithelium; ciliopathy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Humangenetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Nov 2023 14:25 |
| Last Modified: | 07 Nov 2023 14:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56721 |
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