Paddenberg, Eva and Osterloh, Hannah and Jantsch, Jonathan and Nogueira, Andressa and Proff, Peter and Kirschneck, Christian and Schroeder, Agnes (2022) Impact of Leptin on the Expression Profile of Macrophages during Mechanical Strain In Vitro. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (18): 10727. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Childhood obesity is a growing problem in industrial societies and associated with increased leptin levels in serum and salvia. Orthodontic treatment provokes pressure and tension zones within the periodontal ligament, where, in addition to fibroblasts, macrophages are exposed to these mechanical loadings. Given the increasing number of orthodontic patients with these conditions, insights into the effects of elevated leptin levels on the expression profile of macrophages during mechanical strain are of clinical interest. Therefore, the aim of this in vitro study was to assess the influence of leptin on the expression profile of macrophages during simulated orthodontic treatment. RAW264.7 macrophages were incubated with leptin and lipopolysaccharides (LPS) from Porphyromonas gingivalis (P. gingivalis) or with leptin and different types of mechanical strain (tensile, compressive strain). Expression of inflammatory mediators including tumor necrosis factor (TNF), Interleukin-1-B (IL1B), IL6, and prostaglandin endoperoxide synthase (PTGS2) was assessed by RT-qPCR, ELISAs, and immunoblot. Without additional mechanical loading, leptin increased Tnf, Il1b, Il6, and Ptgs2 mRNA in RAW264.7 macrophages by itself and after stimulation with LPS. However, in combination with tensile or compressive strain, leptin reduced the expression and secretion of these inflammatory factors. By itself and in combination with LPS from P. gingivalis, leptin has a pro-inflammatory effect. Both tensile and compressive strain lead to increased expression of inflammatory genes. In contrast to its effect under control conditions or after LPS treatment, leptin showed an anti-inflammatory phenotype after mechanical stress.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ORTHODONTIC TOOTH MOVEMENT; UP-REGULATION; TNF-ALPHA; LPS; OBESITY; ADIPOKINES; TISSUE; CELLS; macrophages; inflammation; orthodontic tooth movement; leptin; mechanical strain |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Nov 2023 14:01 |
| Last Modified: | 14 Nov 2023 14:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56753 |
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