Paddenberg, Eva and Forneck, Anne and Widbiller, Matthias and Smeda, Martyna and Jantsch, Jonathan and Proff, Peter and Kirschneck, Christian and Schroeder, Agnes (2022) Impact of Melatonin on RAW264.7 Macrophages during Mechanical Strain. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (21): 13397. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
The concentration of melatonin is elevated during the night when patients mainly wear removable orthodontic appliances. Next to periodontal ligament fibroblasts and osteoblasts, macrophages react to mechanical strain with an increased expression of inflammatory mediators. Here, we investigated the impact of melatonin on RAW264.7 macrophages exposed to tensile or compressive strain occurring during orthodontic tooth movement in the periodontal ligament. Before exposure to mechanical strain for 4 h, macrophages were pre-incubated with different melatonin concentrations for 24 h, to determine the dependence of melatonin concentration. Afterwards, we performed experiments with and without mechanical strain, the most effective melatonin concentration (25 mu M), and the melatonin receptor 2 (MT2) specific antagonist 4P-PDOT. The expression of inflammatory genes and proteins was investigated by RT-qPCR, ELISAs, and immunoblot. Both tensile and compressive strain increased the expression of the investigated inflammatory factors interleukin-1-beta, interleukin-6, tumor necrosis factor alpha, and prostaglandin endoperoxide synthase-2. This effect was inhibited by the addition of melatonin. Incubation with 4P-PDOT blocked this anti-inflammatory effect of melatonin. Melatonin had an anti-inflammatory effect on macrophages exposed to mechanical strain, independent of the type of mechanical strain. As inhibition was possible with 4P-PDOT, the MT2 receptor might be involved in the regulation of the observed effects.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROMOTES OSTEOBLAST DIFFERENTIATION; MESENCHYMAL STEM-CELLS; NITRIC-OXIDE SYNTHASE; SIGNAL-TRANSDUCTION; SALIVARY MELATONIN; RECEPTORS; BONE; EXPRESSION; RAT; ACTIVATION; macrophages; inflammation; melatonin; mechanical strain |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Nov 2023 06:26 |
| Last Modified: | 27 Nov 2023 06:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56796 |
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