Schweikl, Helmut and Weissenberger, Sarah and Gallorini, Marialucia and Bolay, Carola and Waha, Claudia and Hiller, Karl - Anton and Buchalla, Wolfgang (2022) Influence of HEMA on LPS- and LTA-stimulated IL-6 release from human dental pulp cells. DENTAL MATERIALS, 38 (5). pp. 886-897. ISSN 0109-5641, 1879-0097
Full text not available from this repository. (Request a copy)Abstract
Objective: Dental pulp cells interact with immunogenic components such as LPS (lipopolysaccharide) or LTA (lipoteichoic acid) released from microorganisms in carious lesions. In the present investigation, the formation of the pro-inflammatory cytokines TNF alpha and IL-6 in LPS-or LTA-stimulated cells from the dental pulp interface and pulp fibroblasts was analyzed in the presence of the resin monomer 2-hydroxyethyl methacrylate (HEMA) under varying cellular redox conditions.& nbsp;Method: Human pulp fibroblasts (HPC) or cells from the dental pulp interface expressing an odontoblast phenotype (hOD-1) were exposed to LTA, LPS or HEMA for 1 h or 24 h. Redox homeostasis was modified by the prooxidant BSO (L-buthionine sulfoximine) or the antioxidant NAC (N-acetyl cysteine). Formation of TNF alpha or IL-6 was analyzed by ELISA, and cell survival was determined by a crystal violet assay. Statistical analyses were performed using the Mann-Whitney-U-test.& nbsp;Results: Secretion of TNF alpha was not detected in LPS-or LTA-stimulated HPC or hOD-1, and IL 6 was not found after a short exposure (1 h). After a 24 h exposure, LPS induced a 3-fold increase in IL-6 formation in HPC, while LTA stimulated IL-6 release about 20-fold. Likewise, LTA was more effective than LPS in hOD-1 stimulating IL-6 levels about 50-fold. HEMA inhibited the LPS-and LTA-induced IL-6 release, and this effect was enhanced by BSO but counteracted by NAC in both cell types. IL-6 release was independent of cell survival rates.& nbsp;Conclusions: The protective immune response in odontoblasts and pulp fibroblasts is impaired by monomers such as HEMA through the disturbance of the redox homeostasis. (C)& nbsp;2022 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INDUCED CYTOKINE RELEASE; MACROPHAGES; LIPOPOLYSACCHARIDE; FIBROBLASTS; GLUTATHIONE; INHIBITION; APOPTOSIS; LPS; LTA; Resin monomer; HEMA; Pulp cells; Odontoblasts; Oxidative stress; Cytokine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Prof. Dr. rer. nat. Helmut Schweikl Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Nov 2023 13:49 |
| Last Modified: | 27 Nov 2023 13:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56835 |
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