Schroeder, Agnes and Wagner, Kathrin and Cieplik, Fabian and Spanier, Gerrit and Proff, Peter and Kirschneck, Christian (2023) Impact of phosphorylation of heat shock protein 27 on the expression profile of periodontal ligament fibroblasts during mechanical strain. JOURNAL OF OROFACIAL ORTHOPEDICS-FORTSCHRITTE DER KIEFERORTHOPADIE, 84 (SUPPL). pp. 143-153. ISSN 1434-5293, 1615-6714
Full text not available from this repository. (Request a copy)Abstract
Purpose Orthodontic tooth movement is a complex process involving the remodeling of extracellular matrix and bone as well as inflammatory processes. During orthodontic treatment, sterile inflammation and mechanical loading favor the production of receptor activator of NF-kappa B ligand (RANKL). Simultaneously, expression of osteoprotegerin (OPG) is inhibited. This stimulates bone resorption on the pressure side. Recently, heat shock protein 27 (HSP27) was shown to be expressed in the periodontal ligament after force application and to interfere with inflammatory processes. Methods We investigated the effects of phosphorylated HSP27 on collagen synthesis (COL1A2 mRNA), inflammation (IL1B mRNA, IL6 mRNA, PTGS2 protein) and bone remodeling (RANKL protein, OPG protein) in human periodontal ligament fibroblasts (PDLF) without and with transfection of a plasmid mimicking permanent phosphorylation of HSP27 using real-time quantitative polymerase chain reaction (RT-qPCR), western blot and enzyme-linked immunosorbent assays (ELISAs). Furthermore, we investigated PDLF-induced osteoclastogenesis after compressive strain in a co-culture model with human macrophages. Results In particular, phosphorylated HSP27 increased gene expression of COL1A2 and protein expression of PTGS2, while IL6 mRNA levels were reduced. Furthermore, we observed an increasing effect on the RANKL/OPG ratio and osteoclastogenesis mediated by PDLF. Conclusion Phosphorylation of HSP27 may therefore be involved in the regulation of orthodontic tooth movement by impairment of the sterile inflammation response and osteoclastogenesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ORTHODONTIC TOOTH MOVEMENT; NECROSIS-FACTOR-ALPHA; HSP27 OLIGOMERIZATION; PROSTAGLANDIN E-2; CREVICULAR FLUID; CELLS; OSTEOCLASTOGENESIS; CONNEXIN-43; ADHESION; RELEASE; Heat shock proteins; Orthodontic tooth movement; Compressive forces; Bone remodeling; Osteogenesis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Mar 2024 09:08 |
| Last Modified: | 05 Mar 2024 09:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/56940 |
Actions (login required)
 |
View Item |
