Iacovella, Maria G. and Golfieri, Cristina and Massari, Lucia F. and Busnelli, Sara and Pagliuca, Cinzia and Dal Maschio, Marianna and Infantino, Valentina and Visintin, Rosella and Mechtler, Karl and Ferreira-Cerca, Sebastien and De Wulf, Peter (2015) Rio1 promotes rDNA stability and downregulates RNA polymerase I to ensure rDNA segregation. NATURE COMMUNICATIONS, 6: 6643. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
The conserved protein kinase Rio1 localizes to the cytoplasm and nucleus of eukaryotic cells. While the roles of Rio1 in the cytoplasm are well characterized, its nuclear function remains unknown. Here we show that nuclear Rio1 promotes rDNA array stability and segregation in Saccharomyces cerevisiae. During rDNA replication in S phase, Rio1 downregulates RNA polymerase I (polI) and recruits the histone deacetylase Sir2. Both interventions ensure rDNA copy-number homeostasis and prevent the formation of extrachromosomal rDNA circles, which are linked to accelerated ageing in yeast. During anaphase, Rio1 downregulates polI by targeting its subunit Rpa43, causing polI to dissociate from the rDNA. By stimulating the processing of polI-generated transcripts at the rDNA, Rio1 allows for rDNA condensation and segregation in late anaphase. These events finalize the genome transmission process. We identify Rio1 as an essential nucleolar housekeeper that integrates rDNA replication and segregation with ribosome biogenesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-CYCLE PROGRESSION; RIBOSOMAL DNA-TRANSCRIPTION; SMALL-SUBUNIT PROCESSOME; SACCHAROMYCES-CEREVISIAE; PROTEIN-KINASE; CHROMOSOME SEGREGATION; CYTOPLASMIC MATURATION; CDC14 PHOSPHATASE; IMMUNOPHILIN FPR3; BUDDING YEAST; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jul 2019 12:57 |
| Last Modified: | 22 Jul 2019 12:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5700 |
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