Kropp, Cosimo and Lipp, Julius and Schmidt, Anna Lena and Seisenberger, Christina and Linde, Mona and Hinrichs, Kai-Uwe and Babinger, Patrick (2022) Identification of acetylated diether lipids in halophilic Archaea. MICROBIOLOGYOPEN, 11 (3): e1299. ISSN 2045-8827,
Full text not available from this repository. (Request a copy)Abstract
As a hallmark of Archaea, their cell membranes are comprised of ether lipids. However, Archaea-type ether lipids have recently been identified in Bacteria as well, with a somewhat different composition: In Bacillales, sn-glycerol 1-phosphate is etherified with one C35 isoprenoid chain, which is longer than the typical C20 chain in Archaea, and instead of a second isoprenoid chain, the product heptaprenylglyceryl phosphate becomes dephosphorylated and afterward diacetylated by the O-acetyltransferase YvoF. Interestingly, database searches have revealed YvoF homologs in Halobacteria (Archaea), too. Here, we demonstrate that YvoF from Haloferax volcanii can acetylate geranylgeranylglycerol in vitro. Additionally, we present the first-time identification of acetylated diether lipids in H. volcanii and Halobacterium salinarum by mass spectrometry. A variety of different acetylated lipids, namely acetylated archaeol, and acetylated archaetidylglycerol, were found, suggesting that halobacterial YvoF has a broad substrate range. We suppose that the acetyl group might serve to modify the polarity of the lipid headgroup, with still unknown biological effects.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTACT POLAR LIPIDS; HALOFERAX-VOLCANII; BIOSYNTHESIS; ENZYME; SPECIFICITY; FAMILY; acetyltransferase; archaeol; ether lipids; Halobacteria; mass spectrometry |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Dec 2023 11:13 |
| Last Modified: | 12 Dec 2023 11:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57023 |
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