Bichlmayer, Eva-Marie and Mahl, Lina and Hesse, Leo and Pion, Eric and Haller, Victoria and Moehwald, Andreas and Hackl, Christina and Werner, Jens M. and Schlitt, Hans J. and Schwarz, Siegfried and Kainz, Philipp and Brochhausen, Christoph and Groeger, Christian and Steger, Felix and Koelbl, Oliver and Daniel, Christoph and Amann, Kerstin and Kraus, Andre and Buchholz, Bjorn and Aung, Thiha and Haerteis, Silke (2022) A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices. CELLS, 11 (15): 2269. ISSN , 2073-4409
Full text not available from this repository. (Request a copy)Abstract
(1) Background: Autosomal dominant polycystic kidney disease (ADPKD) is a frequent monogenic disorder that leads to progressive renal cyst growth and renal failure. Strategies to inhibit cyst growth in non-human cyst models have often failed in clinical trials. There is a significant need for models that enable studies of human cyst growth and drug trials. (2) Methods: Renal tissue from ADPKD patients who received a nephrectomy as well as adult mouse kidney slices were cultured on a chorioallantoic membrane (CAM) for one week. The cyst volume was monitored by microscopic and CT-based applications. The weight and angiogenesis were quantified. Morphometric and histological analyses were performed after the removal of the tissues from the CAM. (3) Results: The mouse and human renal tissue mostly remained vital for about one week on the CAM. The growth of cystic tissue was evaluated using microscopic and CT-based volume measurements, which correlated with weight and an increase in angiogenesis, and was accompanied by cyst cell proliferation. (4) Conclusions: The CAM model might bridge the gap between animal studies and clinical trials of human cyst growth, and provide a drug-testing platform for the inhibition of cyst enlargement. Real-time analyses of mouse kidney tissue may provide insights into renal physiology and reduce the need for animal experiments.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHORIOALLANTOIC MEMBRANE; EMBRYO; CT; human renal cystic tissue; ADPKD; chorioallantoic membrane (CAM) model; mouse kidney slices; 3D in vivo model; polycystic kidney disease |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Pathologie Medicine > Lehrstuhl für Strahlentherapie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jan 2024 14:43 |
| Last Modified: | 09 Jan 2024 14:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57133 |
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